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>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-17B. All lots are greater than 98% pure.
Recombinant mIL-17B has a Met on the amino terminus and has a calculated MW of 18,186. DTT-reduced and non-reduced protein migrate as 19 kDa polypeptides. The expected amino-terminal MHPRN of recombinant mIL-17B was verified by amino acid sequencing.
The activity of mIL-17B was assessed by its ability to bind to IL-17RB in a functional ELISA. The concentration at which half-maximal binding was observed for each lot was 0.1-1.0 µg/ml.
Less than 0.01 ng endotoxin/1 μg mIL-17B.